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CABRI:ICLC |
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Accession_number |
ICLC HTL03001
|
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Cell_line_name |
A549
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Brief_description |
Species: human, Caucasian; Tissue: lung; Tumor: carcinoma
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Description |
Species: human, Caucasian male 58 year old; Tissue: lung; Tumor: carcinoma
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Depositor |
Dr L. Lanfrancone, Experimental Oncology Dept, European Institute of Oncology, Milan, Italy
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Bibliographic_reference |
J Natl Cancer Inst 1973;51:1417-1423 [PMID: 4357758
] |
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Morphology |
epithelial, grown as monolayer
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Culture_conditions |
continuous culture; DMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:3-1:4 using trypsin/EDTA; 37°C, 5% CO2
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Viruses |
Search for viruses was not performed
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Properties |
The cells could synthesize lecithin and are positive for keratin
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Release_conditions |
Cell line available for distribution. For non-commercial investigative use only
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Hazard |
-
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Freezing_medium |
Culture medium + 50% FBS + 10% DMSO
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Sterility |
mycoplasma negative, HOECHST and PCR
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Validation_assays |
Multiplex PCR: tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers
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Further_bibliography |
Int J Cancer 1976;17:62-70 [PMID: 175022
]
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